Page 179 - Analytical method for food addtives

Column Reference Detection Mobile phase 15,16 UV at 205 nm Aqueous buffered acetonitrile at Anion exchange, 4.6 pH 6.5, flow rate 1.0 mL/min, mm, 10 µm; × 150 injection volume 100 µL IC-PAK A HC (high capacity) – Waters 17 UV at 210 nm. mM mM NaCl with 1 20 Anion exchange DAD 205–300 nm NaH 2 PO 4 at pH 7 isocratic,

Sample preparation/extraction Extracted from test sample with hot water. Treated with acetonitrile, filtered Deproteinised with acetonitrile, lyophilised and reconstituted in buffer Addition of borax to pH 8.6. Hot water extraction, cleaned and filtered

vegetables and Human plasma

Matrix Meat, baby foods and urine Spinach = suphanilamide = sulphamethizole = sulphadimidine

Method HPLC/IC
(b) IC IC Key: SAM SM SD