Page 21 - Analytical method for food addtives

Analytical methods for food additives
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Column Reference Detection Mobile phase 2 Diode-array mL/min) Gradient elution (1.5 Spherisorb C8 nm at 430 with phosphate buffer containing cetylpyridinium chloride, acetonitrile and methanol 3 nm 520 mL/min) Gradient elution (2 Nova-Pak C18 M using methanol and 0.1 sodium phosphate buffer at pH 7 4 nm 254 mL/min)

Summary of methods for sunset yellow in foods Sample Matrix preparation/extraction Ion pairs with cetylpyridinium Lemonade chloride from aqueous solutions into n-butanol Diluted with water and filtered Bitter Neutralised with aq 50 Fruit juice and centrifuged soy sauce Beverages and foods Neutralised with aq NH 3 and Beve

Table 1.1 (a) Method IP-RP-HPLC RP-HPLC Ion-pair reversed- phase HPLC HPLC HPLC High-performance ion chromatography