Page 24 - Analytical method for food addtives

E110: Sunset yellow 7

Reference 8 9 10 11 12

Detection nm 520 MULTv3.0 Quimio program nm 487 mg nm 485 Between 400 and nm. Partial least 800 squares (PLS) multivariate calibration used

Nova-Pak C18 column with gradient mL/min) with methanol- 7 containing mM tetra-butylammonium bromide Computer program that determines concentration of mixtures of 4 compounds by comparing their spectra The mixture was agitated with 50 Sephadex DEAE A-25gel. The solid phase was extracted and packed into mm cells for spectr

Extraction elution (1.5 phosphate buffer of pH 5 with standard spectra 5 1 determination n-butanol into 1 determination

Sample preparation Diluted with water and filtered Diluted with water and ultrasonicated Filtered food samples were diluted mL with the addition of to 100 M acetate buffer at pH mL 1 5 mL ethanol and 10 Ion-pair formation with octadecyl- trimethylammonium bromide at pH 5.6 Samples dissolved in water and filtered

Matrix Beverages, gelatine, syrups Commercially available dyes Soft drinks, fruit liqueurs and ice-cream Soft drinks Soft drinks, sweets and fruit jellies

Method Ion-pair HPLC Spectro- photometry visible Solid-phase spectrophotometry Spectro- photometric Solid-phase spectrophotometry
(b)