Page 237 - Analytical method for food addtives

Analytical methods for food additives
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Sample preparation/extraction Reference Detection Mobile phase Column 5, 6 UV at 280 nm 5 % acetic acid in H 2 O (A) C18 bonded spherical Antioxidants extracted into acetonitrile. Acetonitrile–methanol (1:1) (preferred) silica or Extract is concentrated and diluted with (B). Gradient. Flow rate: equivalent 2-propano

Table 12.1 contd (b) Matrix Method Oils, fats and HPLC butter oil Oils, foods HPLC and biological fluids RP-HPLC formulations Olive oil HPLC