Page 287 - Analytical method for food addtives

Reference
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moiety recovered, desalted by ion exchange and measured gravimetrically as the barium
constant weight and polysorbate 60 content calculated using gravimetric factor obtained
KOH and acidified; fatty acids extracted with hexane. Aqueous polyol solution desalted
phosphomolybdate complex. The polysorbate 60 content calculated using a gravimetric
Extraction of polysorbate 60 from the sample with suitable solvent. Polysorbate polyol
Polysorbates (PS) were extracted from sample with a mixture of dichloromethane and
Polysorbate 60 extracted with CHCl 3 :ethanol (93+7). Extract saponified with alcohol
polyoxyethylated polyols as insoluble heteropoly acid complex. Precipitate dried to
by mixed-bed ion exchange and barium phosphomolybdate used to precipitate
factor obtained by analysing known amounts of polysorbate 60
by analysing known amounts of polysorbate 60
Principle of method
Summary of methods for polysorbates in foods 192 Analytical methods for food additives ethanol by using an Extrelut column. The extract is further purified by using a silica gel foods column. The PS extract is complexed with cobalt–thiocyanate reagent and was determined spectrophotometrically at 620 nm. PS identity was confirmed by I
Shortening, oils, dressings
Processed
Matrix
Foods

Table 17.1 (a) Method Gravimetric Gravimetric Colorimetric with confirmation by IR, TLC and GC Colorimetric and TLC Colorimetric and TLC