Page 290 - Analytical method for food addtives

E432–6: Polysorbates 193

Reference 7

Detection 620 nm

Method conditions The complex was subjected to HPLC with a GPC column, using a mobile phase of acetonitrile–water (95:5)

Sample preparation Extracted with acetonitrile after rinsing with n-hexane to remove fats and oils. Extract cleaned up using Bond Elut silica gel cartridge (500 mg). Cartridge washed with ethyl acetate. Polysorbates eluted with acetonitrile–methanol (1:2). Eluate treated with cobalt–thiocyanate solution to form a bl

Powdered soup

Matrix

Method HPLC
(b)