Page 310 - Analytical method for food addtives

E472e: Mono/diacetyl tartaric acid esters of mono/diglycerides 207

Reference 1 2 3

Detection UV at 200 nm mM, FID at 250 ºC mm packed Mass spectrometry

Summary of methods for mono/diacetyl tartaric acid esters of mono/diglycerides of fatty acids in foods

Method conditions Nucleosil C18 column at 30 ºC with acetonitrile:(TBA–H 2 PO 4 ) 40 pH 6.5 (15:85) mobile phase at 1 mL/min and 10 µL injection cm × 3 Glass column 150 with 1.5 % silicone SE-30 on 80–100 mesh Chromosorb G at 165 ºC, 5 µL injection at 250 ºC, nitrogen flow 20 mL/min The different products of the more pola

Extraction with ethyl acetate, saponification and acidification of the extract followed by direct injection of a dilute aliquot Extracted under acidic conditions with ethyl acetate, saponified, acidified. Free fatty acids removed with ether and reaction mixture was adsorbed on anion exchange column. Tartaric acid eluted

Sample preparation

Matrix Flour Coffee cream powder Emulsifiers

Table 20.1 Method IP-HPLC GC HPLC–MS