Page 316 - Analytical method for food addtives

E476: Polyglycerol esters of polycondensed fatty acids of castor oil 211

Reference 2 3 4 mn

Detection UV at 220 nm Refractometric UV at 205

Gradient elution with n-hexane and isopropanol at 1 mL/min, Acetonitrile–water (83:17) at Linear gradient elution from 100 % methanol to 100 %

Mobile phase 10 µL injection 1.5 mL/min isopropanol

LiChrosorb Diol Carbohydrate Ultrasphere C18

Column analysis
Summary of methods for polyglycerol polyricinoleate in foods

Sample preparation/extraction Samples dissolved in isopropanol (up to 15 % w/w) Samples dissolved in acetonitrile– water (30:70) Diacylglycerols and unsaturated triacylglycerol standards dissolved in isopropanol and saturated triacylglycerol standards dissolved in dichloromethane

Emulsifiers i.e. polyglycerol esters of fatty Mixtures of polyglycerols Synthetic mixtures of triacylglycerols and diacylglycerols

Matrix acids

Table 21.1 Method HPLC HPLC HPLC