Page 10 - Cascade_Biocatalysis_Integrating_Stereoselective_and_Environmentally_Friendly_Reactions

VIII Contents

8 Racemizable Acyl Donors for Enzymatic Dynamic Kinetic
Resolution 179
Davide Tessaro
8.1 Introduction 179
8.2 The Tools 180
8.2.1 The Enzymes 180
8.2.2 The Racemization of Acyl Compounds 182
8.3 Applications of DKR to Acyl Compounds 183
8.3.1 Base-Catalyzed Racemization 183
8.3.2 DKR of Oxoesters 185
8.3.3 DKR of Thioesters 188
8.4 Conclusions 193
Acknowledgments 194
References 194

9 Stereoselective Hydrolase-Catalyzed Processes in Continuous-Flow
Mode 199
Zolt´an Boros, G´abor Horny´anszky, J´ozsef Nagy, and L´aszl´o Poppe
9.1 Introduction 199
9.1.1 General Remarks on Reactions in Continuous-Flow
Systems 199
9.1.1.1 Stereoselective Reactions in Continuous Flow Systems 202
9.1.1.2 Analytical Applications 203
9.1.2 Nonstereoselective Enzymatic Processes 204
9.2 Enzyme-Catalyzed Stereoselective Reactions in Continuous-Flow
Systems 204
9.2.1 Stereoselective Processes Catalyzed by Nonhydrolytic
Enzymes 204
9.2.2 Stereoselective Processes Catalyzed by Hydrolases 207
9.2.2.1 Applicable Types of Selectivities 207
9.2.2.2 Stereoselective Hydrolytic Reactions 207
9.2.2.3 Stereoselective Acylations 211
9.2.2.4 Effects of the Operation Conditions and the Mode of Enzyme
Immobilization 220
9.3 Outlook and Perspectives 222
References 222

10 Perspectives on Multienzyme Process Technology 231
Paloma A. Santacoloma and John M. Woodley
10.1 Introduction 231
10.2 Multienzyme System Classification 233
10.3 Biocatalyst Options 233
10.3.1 Transport Limitations 235
10.3.2 Compartmentalization 237
10.4 Reactor Options 237