Page 167 - Cascade_Biocatalysis_Integrating_Stereoselective_and_Environmentally_Friendly_Reactions

6.2 Sequential Syntheses 143

HO OH OH
O O
O
O HO R
HO OH NHAc
HO
α1,3GalT
O
OH β1,4GalT HO OH OH HO OH
O
HO O O
HO R O R
NHAc HO OH HO NHAc OHOH
α2,3SiaT HO COOH HO OH OH
H O
Me N O O O O
β1,3GlcNAcT HO HO R
O OH NHAc
OH HO OH OH
O O
O O
HO O HO R
HO OH NHAc
NHAc
β1,4GalT
HO OH OH HO OH OH α2,3SiaT HO OHOH COOH
O O O O O H HO OH OH
O O R O O O
HO OH HO Me N O O
OH HO NHAc NHAc HO R
O OH HO NHAc 2
HO OH OH HO OH OH
O O O O
HO O O O HO R
OH HO NHAc OH NHAc
2
Figure 6.4 One-pot synthesis of poly-LacNAc structures with two recombinant GTs and subsequent action of α2,3SiaT or α1,3GalT yielding dif-
ferent epitopes [85].