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238 10 Perspectives on Multienzyme Process Technology

(a)

(b) (1) (2) (3)

(c)
Figure 10.2 Potential reactor configura- randomly packed using both enzymes immo-
tions. (a) CSTR in series using two solu- bilized, and (3) PBR using one enzyme
ble enzymes. (b) Packed bed reactor (PBR) immobilized and the other enzyme soluble.
configurations using immobilized and sol- (c) Combination of CSTR and PBR in series
uble enzymes. (1) PBR divided in sections using soluble and immobilized enzymes.
using both enzymes immobilized, (2) PBR

(e.g., plug flow, well-mixed), and/or different conditions (e.g., pH, T). An alternative
approach is to use a single reactor with variations in conditions dependent upon
position (spatial variation) or time (temporal variation) in the reactor. In both
strategies, the extra flexibility needs to be addressed in process development.