Page 272 - Color Atlas of Biochemistry

Genetic engineering 263

A. Polymerase chain reaction (PCR) B. DNA electrophoresis

Electrophoresis chamber Sample
DNA
a Heat

b Hybridize
Cycle
Primer 1
Power supply
De-novo synthesis unit
c (DNA polymerase)

Agarose gel

a b c
H C
3
Cycle Br N
2
NH 2
Ethidium bromide

1 2 3
bp
a b c

b 1857
Cycle
3 1058
929
a

383
etc

C. Overexpression of proteins

Ribosome Gene to be Host cell
binding expressed
site Trans-
formation

Repli-
Inducible cation Induction
promotor

Origin of Gene for
replication antibiotic
resistance Overexpressed Protein Cell
protein purification lysis
Expression plasmid

Koolman, Color Atlas of Biochemistry, 2nd edition © 2005 Thieme
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