Page 274 - Color Atlas of Biochemistry

Genetic engineering 265

A. DNA fingerprinting B. Diagnosis of sickle-cell anemia using RFLP

STR, z.B.
DNA TCTATCTGTCTG Mst II Mst II
1200 bp 201 bp
Extraction Normal
gene
E1 I1 E2
Sickle-cell
gene
1401 bp
Evidence Mst II Mst II
PCR

Mutation
Locus 1 1.4
Locus 2 1.2 1 Normal (A/A)
2 Heterozygotic (A/S)
Locus 3 1 2 3
kbp 3 Homozygotic (S/S)

Amplificate Comparative
fragments C. Evidence of viral DNA using RT-PCR
RNA Hy- ss- ds-
brid DNA DNA Amplificate Standards

P2
Alleles
Virus
1/3 Locus 1
1/4 PCR
Locus 2 P3
2/4
P1
3/3
Locus 3 Agarose gel
3/5
Reverse DNA polymerase
Agarose gel transcriptase

D. Gene therapy
Tumor- Viral vector
Viral vector with
foreign DNA specific with foreign DNA
receptor

Normal Tumor- Tumor cell
body cell specific
DNA promoter DNA

mRNA E1 mRNA
Harmless
product Immune
Gene E1 system
product Gene
product
E1
Accu- Cytostatic
mulates
agent
A B E1 C

Apoptosis
E2 Genome
Toxic Antisense Cell Cell
product DNA Lipo- proliferation death
some

1. For metabolic defects 2. For tumors

Koolman, Color Atlas of Biochemistry, 2nd edition © 2005 Thieme
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