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436                                                                            Afshin Tatar


                   with the cell attachment. Even in case of release of dying of cells, the biopolymers
                   will be left behind [792].
                   Fractured carbonate reservoir contains most of the world’s oil [793]. In this type of
                reservoirs, the matrix blocks are mixed to oil-wet. This characteristic leads to difficult
                water absorption onto the matrix blocks, which in turn diminishes the sweep effi-
                ciency. In fact, the oil trapped in this region will be unaffected by the floodwater
                [15]. It is proved that the oil recovery can be improved by turning the matrix surface
                to a more water-wet condition [16]. As it was mentioned before, this can be done by
                introducing surfactants, enzymes, or development of a biofilm on the reservoir rock
                surface by microorganisms. Wettability alteration plays a crucial role in oil and surfac-
                tant adsorption in carbonates and clays [10]. In spite of carbonates, wettability of the
                mineral surfaces plays a secondary role in oil displacement in sandstone formations.
                Thus, wettability reversal by microbial activity will not provide a promising mecha-
                nism for enhancing oil recovery in sandstone formations [66]. However, when the
                hydrocarbon degrading microorganisms coat the oil droplets with a biofilm, the role
                of wettability may become trivial [771,794]. Karimi et al. [715] through their experi-
                ments concluded that the effect of biofilm formation was much more significant com-
                pared with many other microbial products including biosurfactants in enhancement of
                wettability. It is reported that brine composition affects the wettability changes
                [795 797] (Fig. 10.8).

                10.11.7 Emulsification
                Microorganisms are capable to produce bioemulsifiers (high molecular weight biosur-
                factants). These biochemicals create micro-emulsion in which the water is solubilized
                in hydrocarbon or hydrocarbon is solubilized in water. Further details are provided in
                Section 10.10.1.





















                Figure 10.8 MEOR by modifying the wettability [31]. MEOR, microbial enhanced oil recovery.
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