Page 32 - Macromolecular Crystallography

CLASSICAL CLONING, EXPRESSION, AND PURIFICATION 21

Table 1.3 Affinity chromatography applications immobilised mini-chaperones Proc. Nat. Acad. Sci. USA
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Ligands Target molecules Examples
Amann, E., Brosius, J., and Ptashne, M. (1983). Vectors
bearing a hybrid trp-lac promoter useful for regulated
Lectins α-D-glucopyranosyl Adenosine deaminase
expression of cloned genes in Escherichia coli. Gene 25,
Concanavalin A α-D-mannopyranosyl γ-Glutamyl transferase
167–178.
Heparin Nucleic acid binding DNA helicases
Appelbaum, E. and Shatzman, A. R. (1999). Prokaryotes in
proteins Restriction
vivo expression systems. In: Protein Expression Practical
endonucleases
Approach, Higgins, S. J. and Hames, B. D., eds. Oxford
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University Press.
Growth factors Endothelial cell GF
Ni-NTA His 6 -tag N- or C-terminus (His)6 Ayres, M. D., Howard, S. C., Kuzio, J., Lopez-Ferber, M.,
Calmodulin Calmodulin- ATPase and Possee, R. D. (1994). The complete DNA sequence
dependent of Autographa californica nuclear polyhedrosis virus.
enzymes Virology 202, 586–605.
Cohen, S. L. and Chait, B. T. (2001). Mass spectrometry as
ssDNA DNA-dependent DNA-polymerases
a tool for protein crystallography. Annu. Rev. Biophys.
enzymes alkyl transferase
Biomol. Struct. 30, 67–85.
+
+
Triazine dyes: NAD , NADP - Dehydrogenases
Cregg, J. M., Vedvick, T. S., and Raschke, W. C.
Cibacron blue dependent Quinone reductases
(1993). Recent advances in the expression of for-
enzymes
eign genes in Pichia pastoris. Bio/Technology 11,
5 -AMP ATP-dependent cAMP-dependent

905–910.
enzymes protein kinase
Creighton, T. E. (1986). Disulphide bonds as probes of
Protein A Immunoglobulins Fc region of IgGs
protein folding pathways. Method Enzymol. 131, 83–106.
MAB Antigens Protein A fusions
Davis, S. J., Puklavec, M. J., Ashford, D. A., Harlos, K.,
Glutathione Glutathione S Glutathione S
Jones, E. Y., Stuart, D. I., and Williams, A. F. (1993).
transferases transferase fusions
Expression of soluble recombinant glycoproteins with
Amylose Maltose binding MBP fusions
predefined glycosylation: application to the crystalliza-
protein
tion of the T-cell glycoprotein CD2. Protein Eng. 6,
229–232.
Modified from Skelly and Madden (1996). In: Crystallographic Methods and
Protocols, Jones, C., Mulloy, B. and Sanderson, M. R., eds. Humana Press. Edwards, C. P. and Aruffo, A. (1993). Current applications
of COS cell based transient expression systems. Curr.
Opin. Biotechnol 4, 558–563.
post-translational modification can be detected by
Felgner, P. L., Gadek, T. R., Holm, M., Roman, R., Chan,
electrospray mass spectrometry (ESI-MS) which is
H. W., Wenz, M., Northrop, J. P., Ringold, G. M., and
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assisted laser desorption ionization (MALDI) is a lipid-mediated DNA-transfection procedure. Proc. Nat.
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used in the presence of buffers and detergents. Cap- Geisse, S., Gram, H., Kleuser, B., and Kocher, H. P. (1996).
illary electrophoresis (CE) is another powerful tool Eukaryotic expression systems: a comparison. Protein
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Gerard, R. D. and Gluzman, Y. (1985). New host cell system
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for regulated simian virus 40 DNA replication. Mol. Cell
a single-charge difference. Dynamic light scattering
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(DLS) can provide evidence of aggregation and the
Graham, F. L. and van der Eb, A. J. (1973). Anew technique
oligomeric state of a protein, which can be a helpful
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Jacob, F. and Monod, J. (1961). Genetic regulatory mech-
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