Page 105 - Modern Derivatization Methods for Separation Sciences

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shows stronger toxicity, however, carcinogenicity and teratogenicity are not known at present.

Fig. 1.2.18.
Schematic diagram of on-column derivatization
HPLC with automated sample clean-up system.

Fig. 1.2.19.
Typical chromatograms of amines from: (A) standards; (B) anchovy; and (C) dry sausage. Standards:
Him (4 ng), Agm (4 ng), Tym (10 ng), Dap (40 ng), Put (20 ng), Spd (20 ng), Spm (80 ng), Cad
(20 ng), (Reproduced from ref. 261: J. Food Hyg. Soc. Japan, (1995) 36, p. 640. Fig. 1.).

For the analysis of alkyltin, ECD-GC and FPDGC are the main methods in use and HPLC is less used.
Sample preparation for HPLC is simpler than that for the GC method. The use of HPLC for the analysis
of alkyltin has been reported after alkali decomposition. Bis(tri-n-butyltin) oxide (TBTO) in fish was
converted to trin-butyltin chloride(TBTC) with hydrochloric acid,

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