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tetrodotoxin (TTX) are caused by accidental intake of toxic parts of the pufferfish such as the liver.
TTX is known to give a fluorescent 2-amino-quinazoline derivative when heated in a strong alkaline
solution [235]. This reaction is applied for post-column HPLC method [236,237]. The analytical
procedure includes extraction of TTX with diluted acetic acid, defattening with diethyl ether, clean-up
with Amberlite CG-50 and separation with cation-exchange or reversed-phase HPLC. Post-column
reaction employs 4N-NaOH solution and fluorescent derivatization is carried out in boiling water
(λex357 nm, λem510 nm). The detection limit is 5 ng/injection and the HPLC method is far more
sensitive and specific than mouse bioassay. This method was useful for investigating naturally
occurring TTX analogues in puffers and other animals [237,238].

Ciguatoxin
Ciguatera is a term given to a peculiar form of poisoning caused by ingestion of fish that live mainly in
coral reef areas. Ciguratoxin (CTX) has a primary alcohol group reactive to fluorescent reagents [239].
Reaction with 1-anthrylcarbocyanide produced a fluorescent ester of CTX, which was successfully
determined by

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