Page 102 - Modern Derivatization Methods for Separation Sciences

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is 1-20 ppm) [244-246]. Pre-column derivatization with FMOC was applied to the analysis of tyramine,
βphenylethylamine and tryptamine in chicken with UV detection [247] and amines in wine, fruit or
vegetable juice, fish, cheese and amino acids with fluorescent detection (λex265 nm, λem315 nm) [74].
These fluorescent derivatization reagents themselves and their hydrolysis products such as 9-
fluorenylmethanol have strong fluorescence, and thus the reagents are used for only precolumn
derivatization in the HPLC method. After the derivatization reaction, excess reagents should be
removed by addition of proline for DNS, heptylamine for FMOC or extraction by pentane. Bis-
substituted derivatives of histamine

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