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Fig. 2.45.
Reaction of reagent MAMA with airborne csocyanates.

studied range of concentrations of prospidin is 1-50 µg/ml.

DDTC was also used for the HPLC determination of busulfan, OH compound, in human serum [150].

2.7.3—
9-methylamino-methylanthracene (MAMA) [151]

MAMA is a secondary amino compound commonly used for derivatization and quantitation of specific
commercial isocyanate compounds by HPLC with fluorescence and UV light absorbance. The
determination of airborne total reactive isocyanate group compounds is of interest in industrial hygiene.
Detection is by UV absorption at 370 nm. MAMA produces substituted urea in the reaction with
airborne isocyanate monomers (Fig. 2.45).

References

[1] Lawrence, J.F. and Fri, R.W. (1976) Chemical Derivatization in Liquid Chromatography, Else-
vier, Amsterdam, Netherlands.

[2] Frei, R.W., in Fri, R.W. and Lawrence, J.F. (Eds) (1981) Chemical Derivatization in Analytical
Chemistry, Vol. 1, Chromatography, Plenum Press, New York.

[3] Knapp, D.R. (1979) Handbook of Analytical Derivatization Reactions, J. Wiley and Sons, New
York.

[4] Blau, K. and King, G.S. (Eds) (1977) Handbook of Derivatives for Chromatography, Heyden,
London.

[5] Meulendijk, J.A.P. and Underberg, W.J.M. (1990) in Detection Oriented Derivatization Techniques
in Liquid Chromatography, H. Lingeman and W.J.M. Underberg (Eds), pp 247-281, Marcel Dekker,
New York.

[6] Li, F. and Lim, C.K. (1993) in Handbook of Derivatives for Chromatography, 2nd ed, K. Blau and
J. Halket (Eds), pp 158-174, John Wiley & Sons, Chicheste, New York, Brisbane, Toronto and
Singapore.

[7] Szule, M.E. and Krull, I.S. (1994) J. Chromatogr. A, 659, 231.

[8] Krull, I.S., Deyl, Z. and Lingeman, H. (1994) J. Chromatogr. B., 659, 1.

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