Page 203 - Modern Derivatization Methods for Separation Sciences

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Fig. 2.40.
The reaction of 2-halopyridinium compounds and thiols.

Fig. 2.41.
Typical chromatograms of captopril (50 mg oral
dose) in human plasma as BCPB derivative. (a)
Blank, (b) patient sample. Broken arrow represents
captopril and solid arrow represents the internal
standard. [Reproduced from ref. 141, p. 288, Fig. 3.].

314 nm. Oxidized and protein-bound captopril was converted to reduced form using triphenylphosphine
and derivatized and quantified in the same manner. The detection limit was 0.3 ng/ml and the
calibration range was 10-500 ng/ml.
BCPB Application: Determination of Captopril in Human Plasma [141]

Human plasma was deproteinized using 3 M perchloric acid and neutralized with 1 M NaOH. To the
solution, 1 M Tris buffer (pH 8.2) and 20 mg/ml BCPB solution were added and derivatized for 15 min.
After acidifying by adding 4 M phosphoric acid, the reaction mixture was centrifuged and the derivative

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