Page 40 - Modern Derivatization Methods for Separation Sciences

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Clean-up: Liquid-liquid extraction

Detection: Fluorescence

Analytical parameters: Range, 1-100 ng/ml for propranolol; 2-50 ng/ml for 4-hydroxypropranolol
enantiomers, using 0.5 ml of human plasma.

Anti Arrhythmia Agents

Enantioselective determination of diprafenone in human plasma [32].

Derivatization: Precolumn derivatization with homochiral R(-)-1-(1-naphthyl)ethylisocyanate

Clean-up: Liquid-liquid extraction

Detection: UV, 220 nm

Analytical parameters: LOQ, 10 ng/ml for S(-)and R(+)-diprafenone in plasma.

Cardiotonic Agents

Digoxin and its metabolities in human serum [33].

Derivatization: Precolumn fluorescence derivatization with 1-naphthoyl chloride in the presence of
4dimethylaminopyridine

Clean-up: Cyclodextrin and Cl solid-phase extraction

Detection: Fluorescence

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