Page 35 - Modern Derivatization Methods for Separation Sciences

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Fig. 1.1.7.
Typical chromatograms obtained from A;
blank rat plasma and B; spiked plasma containing
10 µg/ml racemic ibuprofen. Peaks are (-)APMB
amides of 1; S-ibuprofen, 2;R-ibuprofen and 3;
internal standard. [Reproduced from ref. 13, p.
173, Fig. 2.].

Fig. 1.1.8.
Derivatization reaction of ivermectin. [Reproduced from ref. 16, p. 74, Scheme.].

chromatography with fluorescence detection. The method involves dehydrative aromatization using
non-fluorescent reagents.

Ivermectin has two hydroxyl groups on the dihydroxycyclohexene ring (Fig. 1.1.8). The hydroxyl
groups are sterically positioned to allow for the easy elimination of water to produce a delocalized
electron system: i.e., heating with acetic anhydride in pyridine causes the elimination of two moles of
water, producing a fluorophore

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