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Analytical parameters: Detection limit of 5-FU in hair, 0.01 ppm.

Cisplatin in human blood [25]. Derivatization: Precolumn derivatization with bis (salicylaldehyde)
tetramethylethylenediimine

Clean-up: Liquid-liquid extraction with chloroform

Detection: UV, 254 nm.

Methotrexate in plasma [26].

Derivatization: On-line precolumn oxidative cleavage with Cerium (IV) trihydroxyhydroperoxide
(CTH) as a packed oxidant to form 2,4diaminopteridine derivatives

Clean-up: Protein precipitation and solvent purification

Detection: Fluorescence detection, Ex. 367 nm and Em. 463 nm

Analytical parameters: Coefficients of variance, <4%; mean relative errors, 1.11%-7.83%; recovery,
93.74%-98.11%.

Cremophor EL in plasma [27].

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