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264 Multidimensional Chromatography

Figure 11.4 Chromatograms of plasma samples on a silica–chiralcel OJ coupled column
system: (a) plasma spiked with oxprenolol (internal standard); (b) plasma spiked with
0.40 g/ml metyrapone and 0.39 g/ml metyrapol (racemate); (c) plasma sample obtained
after oral administration of 750 mg metarypone. Peaks are as follows: 1, metyrapone; 2,
metyrapol enantiomers; 3, oxprenolol. Reprinted from Journal of Chromatography, 665, J. A.
Chiarotto and I. W. Wainer, ‘Determination of metyrapone and the enantiomers of its chiral
metabolite metyrapol in human plasma and urine using coupled achiral–chiral liquid chro-
matography,’ pp. 147–154, copyright 1995, with permission from Elsevier Science.