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Biomedical and Pharmaceutical Applications                      271




























































                           Figure 11.7 (a) Set-up used for immunoaffinity SPE–LC. (b)  The analysis of   -19-
                           nortestosterone ( -19-NT) and  -19-nortestosterone ( -19-NT) (300 ng/l each) in 26.5 ml
                           calf urine by using on-line SPE–LC with an immunoaffinity pre-column against  -19-NT.
                           Norgestrel (NG) is used for desorption of the immunoaffinity pre-column. Reprinted from
                           Journal of Chromatography, 452, A. Farjam et al., ‘Immunoaffinity pre-column for selective
                           on-line sample pre-treatment in high-performance liquid chromatography determination of
                           19-nortestosterone,’ pp. 419–433, copyright 1988, with permission from Elsevier Science.
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