Page 329 - Multidimensional Chromatography

Industrial and Polymer Applications 319

Figure 12.12 Coupled SEC–RPLC separation of Plioflex rubber stock: (a) SEC; (b) RPLC
trace of fraction 1, Wingstay 100 (Five-peak pattern is representative of diarylphenylenedi-
amine isomers); (c) RPLC trace of fraction 2, mixed disulfide and MBTS (2,2´-thiobis (ben-
zothiazole)). Obtained under the same conditions as given for Figure 12.11. Reprinted from
Journal of Chromatography, 149, E. L. Johnson et al., ‘Coupled column chromatography
employing exclusion and a reversed phase. A potential general approach to sequential analy-
sis’, pp. 571–585, copyright 1978, with permission from Elsevier Science.

and higher peak intensities for trace components to be obtained, as can be seen in
Figures 12.13(a) (with trapping) and 12.13(b) (without trapping).
Multi-column switching can be an effective approach for the determination of
high and low concentrations of sample components in complex mixtures. This is a
very powerful technique for the analytical and preparative separation of components