Page 363 - Multidimensional Chromatography

354 Multidimensional Chromatography

Figure 13.10 LC–LC chromatogram of a surface water sample spiked at 2 g l 1 with
atrazine, and its metabolites (registered at 220 nm). Conditions: volume of sample injected,
2 ml; clean-up time, 2.60 min; transfer time, 4.2 min; The blank was subtracted. Peak identi-
fication is as follows: 1, DIA; 2, HA; 3, DEA; 4, atrazine. Reprinted from Journal
of Chromatography, A 778, F. Hernández et al., ‘New method for the rapid determination of
triazine herbicides and some of their main metabolites in water by using coupled-column liq-
uid chromatography and large volume injection’, pp. 171–181, copyright 1997, with permis-
sion from Elsevier Science.

possible key compounds in photochemical reactions. In this example (53), 13 differ-
ent aldehydes and ketones were determined in air and water samples by using cou-
pled-column RPLC. These compounds were determined by UV detection of the
derivative formed with 2,3-dinitrophenylhydrazine in C 18 SPE cartridges. With two