Page 515 - Book Hosokawa Nanoparticle Technology Handbook

17 ADDRESSING OF NANOPARTICLES BY USING DNA MOLECULES APPLICATIONS

Patterning of DNA molecules electric field, because the force by the AC electric
field arises by polarization of DNA molecules.
Therefore, gap between electrodes is shortened and
electric field strength is intensified for AC stretch-
ing operation [2].
As another method, a stretching method based on
Stretching and Fixation on substrate movement of interface between ice and solution was
also proposed [3]. DNA molecules tend to be
excluded into the solution area. Therefore, if the ter-
minus of DNA molecules is anchored on substrates or
Sequence specific binding of
nanotubes or nanoparticles in ice crystal during unidirection freezing of solution
with DNA, the remaining parts of those DNA are
stretched (Fig. 17.4d).
Binding of nanotubes or nanoparticles
2. Addressing of nanoparticles

Holiday junction arrays were designed and applied for
addressing of nanoparticles [4, 5]. When one strand of
DNA is replaced with conjugate DNA–gold particles,
the gold particles are aligned along the holiday junc-
tion arrays.
Figure 17.3 Further development of addressing method can be
Functional structure based on nanoparticles aligned by expected if positions and shapes of DNA molecules
DNA hybridization. can be manipulated on the substrate. This addressing

a) A method based on a meniscus movement (molecular combing)
Movement

b) A method based on electric field
Coulomb force

OFF ON ON
c) A method based on flow
Flow

d) A method based on movement ice/water interface
Interface
Frozen area movement
Coo Coo
-ler -ler

Solution area

Figure 17.4
Various methods for DNA stretching.

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