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Laukó et al. [13] developed a GC/MS procedure for the analysis of crude cholesterol. Crude cholesterol
is obtained from the spinal cord and brain of cattle and from wool grease. It is used in the
pharmaceutical and cosmetic industries as an excipient in the production of ointments and creams, as an
intermediate in hormone synthesis and in the manufacture of vitamin D3 and liquid crystals. The gas
chromatograph employed was the Hewlett-Packard 5890 using a capillary column 25 m long, 320 µm
I.D, carrying a film of phenylmethylsilicone gum 0.52 µm thick and a 501 split injection system. The
column was operated at 280°C and the detector and transit lines at 300°C. The internal standard was
testosterone propionate. I µl samples of a 5% w/V solution of the crude cholesterol were injected. The
mass spectrometer was the VG-TRIO-2 quadrupole used in the positive ion electron impact mode with
an ionization energy of 70 eV, a total ion current chromatogram of the separation is shown in figure
5.16.
Figure 5.16.
Total Ion Current Chromatogram
of Crude Cholesterol (ref 13)
