Page 488 - Tandem Techniques
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in an oven at 40°C for 30 seconds to remove the dichloromethane, and the contents of each cup ware
then examined by diffuse reflectance.
Figure 12.11
Diagram of TLC Plate Attached to the Strip
Plate for the Transfer of the Separated Spots
Reprinted with permission from K. H. Shafer, P. R. Griffiths and W. Shu-Qin,
Anal. Chem., 58(1986)2708, Copyright 1986 American Chemical Society.
After the IR measurements have been made, acetone was passed through the cups to clean them. The
diffuse reflectance measurements were carried out on an Analect FX-6200 FT-IR, equipped with a
diffuse reflectance accessory. The strip was moved with a standard x/y drive mechanism. The location
of each component was found by monitoring the individual spectra and/or the reconstructed
chromatogram. The spectrum was taken at the most concentrated portion of the peak at 4 cm
-1
resolution.
The percentage of infrared radiation reflected from the stationary phase of a TLC plate is significantly
less than that reflected from an IR-
