Page 506 - Tandem Techniques
P. 506
Page 492
An example of the use of the CE/MS tandem system is afforded by the example given in Figure 13.6.
The two chromatograms obtained by single ion monitoring were from a solid phase extract of urine
from a female patient who had been treated with 0.5 mg/day of Haloperidol.
Figure 13.6
The Chromatogram Obtained by Single Ion Monitoring from a CE/MS Analysis of
Haloperidol and its Metabolite Contained in an Urine Extract.
The capillary zone electrophoresis conditions were 50 mM NH4OAc containing 10% methanol and 1%
of acetic acid separated at 15 kV in an uncoated silica capillary (65 cm long and 50 µm I.D.). The
sheath liquid for the electrospray interface was a mixture of isopropanol, water and acetic acid (60:40:1)
and the electrospray voltage was -3.4 kV. The eluents were scanned from 50 to 400 D at 2 sec/decade
and a resolution of 1200. The ions at 376 and 380 corresponded to unmetabolized Haloperidol and
reduced metabolite respectively.
Capillary Electrophoresis Operated in Tandem with the Magnetic Sector Mass Spectrometer
Capillary electrophoresis has also been operated in tandem with a magnetic sector mass spectrometer by
Perkins and Tomer [9], who employed both bare fused silica tubes, and those with surfaces carrying 3-
