Page 507 - Tandem Techniques
P. 507
Page 493
(aminopropyl)-trimethoxysilane derivatives, for the electrophoretic separation. The tandem system was
used to separate some simple peptide mixtures and the small proteins that naturally occur in snake
venom. A potential problem encountered in the electrophoretic separation of the basic peptides and
proteins results from the net positive charge that they acquire, even in solvents at high pH values. This
leads to their retention on the negatively charged column walls, and consequent peak broadening. If the
walls of the tube are derivatized with 3-(aminopropyl)trimethoxysilane, then the amino-
propyldimethoxy groups on the column surface will cause the surface charge to become positive.
Figure 13.7
Modified Probe for Capillary Electrophoresis Interface
with Magnetic Sector Mass Spectrometer
