Page 186 - The Biochemistry of Inorganic Polyphosphates
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WU095/Kulaev
               WU095-08
                                     Peculiarities of polyphosphate metabolism
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                            Figure 8.28 Changes in the content of PolyP fractions during growth of Neurospora crassa strains
                            ad-6 (parent strain) and 30,19-3 (a leaky mutant in exopolyphosphatase), and a slime mutant devoid
                            of the cell envelope (Trilisenko et al., 1980; 1982a,b): (◦) growth; (•) PolyP content in different
                            fractions.

                            magnesium as the predominant inorganic components (Komine et al., 2000). These or-
                            ganelles were similar to acidocalcisomes of other microoganisms (Ruiz et al., 2001b). X-ray
                            microanalysis of the electron-dense vacuoles or PolyP bodies of C. reinhardtii showed large
                            amounts of phosphorus, magnesium, calcium and zinc. Immunofluorescence microscopy
                                                                      +
                            revealed a vacuolar-type proton pyrophosphatase (H –PPase) in this compartment. Purifi-
                            cation of the electron-dense vacuoles using iodixanol density gradients showed preferential
                                                        +
                                          +
                            localization of H –PPase and V–H –ATPase activities, in addition to high concentrations
                            of PP i and short- and long-chain PolyPs (Ruiz et al., 2001b).
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