Page 34 - The Biochemistry of Inorganic Polyphosphates

March 9, 2004
WU095/Kulaev
WU095-02
—
— 15:25 8–12 Char Count= 0 — — — —
n 29–35 37–46
Clark (1986) % — 2.4 — 47.6 — 31.6 — — 18.4 100
cells. a,b of Method al. et PolyP — 406 — 7940 — 5265 — — 3062 673 16 980 33

cerevisiae P i — 3166 — 96 — — — — —

S.
from n 8–26 — 20–28 — — — — 50–62 —

isolated and modification (1971) al. % 28.9 — 15.5 — — — — 34.8 20.8

PolyPs Langen of with et 100

of Chernychova PolyP 5857 — 3131 — — — — 7057 4203 248 980
(n) Method (1959), Liss of 20 33
polymerization P i 2890 — — — — — — — —

of and n 8–26 — 20–28 — 32–37 — 50–55 — —
degree modification (1960) % 28.7 — 15.3 — 26.0 — 23.3 — 6.7 100

and Langen of with al. et
biomass) Method (1959), Kulaev of PolyP 5857 — 3131 — 5323 — 4759 — 1358 428 20 980 33

dry Liss P i 2890 — — — — — — — —
(µgP i /g 30 ◦ C, a The data presented are taken from triplicate experiments. c EDTA, ethylenediaminetetraacetic acid; SDS, sodium dodecyl sulfate.

Content ◦ C; 30 ◦ C; 20 ◦ C; min; 60 7–8; pH min ◦ C, 0 9–10; III PolyP 7–8; pH phenol– min 5 ◦ C; 0 12; PolyP(IV) 0 7.4; ◦ C; PolyP(V)

2.1 HClO 4 ;0 PolyP(I) Trichloroacetic acid–acetone; min PolyP(II) EDTA; ◦ C; 3 pH min; EDTA; ◦ C; chloroform; pH pH SDS; min HClO 4 ;90 min; PolyP P i b ‘—’ indicates not detected.
Table Fraction c N 0.5 min; % 2 3 NaClO 4 ;0 mM 2 20 NaOH; 30 mM 2 20 NaOH; min; % 1 30 N 0.5 40 Total Total

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