Page 249 - Vibrational Spectroscopic Imaging for Biomedical Applications
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CHAPTER 8
Raman Microscopy for
Biomedical Applications:
Toward an Efficient
Diagnosis of Tissues,
Cells, and Bacteria
Christoph Krafft, Ute Neugebauer
Institute of Photonic Technology
Jena, Germany
Jürgen Popp
Institute of Physical Chemistry
University Jena
Jena, Germany
he advantages of Raman spectroscopy to diagnose tissue, cells,
and bacteria include that the method provides a wealth of
Tinformation about the molecular structure and biochemical
composition without labeling. The disadvantages such as low sensi-
tivity and overlapping autofluorescence have been overcome by bet-
ter instrumentation and excitation with near-infrared lasers. This
contribution summarizes recent research results on fiber-optic Raman
spectroscopy of tissue, Raman imaging of tissue and cells, and Raman
spectroscopy of bacteria. The sections are organized from low-spatial
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