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264                              Advances in Eco-Fuels for a Sustainable Environment


                                   Rice bran


                                  Non catalytic
                             in situ trans(esterification)

                                               Upper layer
                                Solvent extraction      Vacuum evaporator

             Water phase  Filtrate
              containing
              fermentable       Vacuum filtration         Crude biodiesel
                sugars                   Solid

                                Defatted rice bran        Extraction of
                                                         unreacted oils and
                                                        bioactive compounds
                                                        using deep eutectic
                     Carbohydrate
                                                          solvent (DES)
                                              Purified
                                              biodiesel
                       Protein
                                                       Recovery of bioactive
                     Phenolic and                     compounds (γ-oryzanol)
                  flavonoid compounds

                      Fiber, etc.                       Bioactive compounds
                                                           (γ-oryzanol)

         Fig. 9.3 The flow diagrams of value-added products (biodiesel and γ-oryzanol) produced from
         rice bran.


         compounds (g-oryzanol) from the DES phase will be easier because γ-oryzanol was
         enriched in this phase. Consequently, by using the biorefinery concept as shown in
         Fig. 9.3, the production cost of biodiesel can be lowered.



         9.4.2 Biorefinery approach in biodiesel production from
                microalgae
         Microalgae have been touted as a potential feedstock not only in biofuel production
         but also as a source of bioactive compounds. Even though the lipid content in the
         microalgae cell is quite high, only using the microalgae lipid to produce biodiesel
         is still not economically feasible. Valuable product generation from other constituents
         of the microalgae cell could be a possible way to increase the profit of the product
         output [27]. Although the composition of the cell depends on the microalgae species
         and the cultivation conditions, many valuable products can be obtained from cells.
         Constituents of microalgae can be divided into major components and minor compo-
         nents. The major components are lipids, proteins, and carbohydrates while the minor
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