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8                                Advances in Eco-Fuels for a Sustainable Environment


          Table 1.3 List of the spaces used for 3G biofuel production in the literature

                               Oil content
                               per tonne of
                               biomass       Lipid      Lipid
                Name of        (w t % dry    content (%,  productivity
                microorganisms  mass)        w/w dw )   (mg/L/day)  References
          1.    Neochloris     35.00–65.00   29.00–65.00  90.00–134.00  [71, 72]
                oleoabundans
          2.    Chlorella      63.20         5.00–58.00  11.20–40.00  [73]
                vulgaris
          3.    Crypthecodium  56.00         20.00–51.10  –         [74]
                cohnii
          4.    Nannochloropsis  50.00       22.70–29.70  84.00–142.00  [75]
                oculata
          5.    Monodus        39.30         16.00      30.40       [71]
                subterraneus
          6.    Cylindrotheca sp.  16.00–37.00                      [76]
          7.    Phaeodactylum  20.00–30.00   18.00–57.00  44.80     [74]
                tricornutum
          8.    Tetraselmis    15.00–23.00   8.50–23.00  27.00–36.40  [74]
                sueica
          9.    Chlorella      22.00         19.00–22.00  44.70     [71, 77]
                sorokiana
          10.   Dunaliella salina  14.00–20.00  6.00–25.00  116.00  [67]
          11.   Porphyridium   19.30         9.00–18.80  34.80      [71]
                cruentum

            The microorganism or 3G biofuels are mainly produced from microalgae biomass,
         which has some remarkable advantages such as self-productivity, fast growing,
         require less water, and can grow in undeveloped land [33]. However, the key factor
         in extracting biofuel is the lipid content. For instance, Scott and Davey [67] investi-
         gated the Chlorella protothecoides, Chlorella vulgaris, Dunaliella salina, and
         Chamydomonas reinhardtii species and found about 60%–70% lipid content in them.
         Chen and Yeh [68] reported higher productivity of lipids in Chlorella vulgaris of
         about 7.4g/L/day. This feedstock can also be used to produce bioethanol, jet fuel,
         or aviation biogasoline [69]. In addition, some authors also studied other 2G feed-
         stocks such as pongamia, sugarcane molasses, waste cooking oil, jatropha, camelina,
         and tallow for aviation biofuel production [6, 70] (Table 1.3).



         1.4   Significance of using ecofuel

         There has been ongoing interest in both the road and aviation transport sectors in
         developing new and alternative fuels for future energy security and a sustainable envi-
         ronment. For instance, the aviation industries are struggling to find new and
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