Page 99 - Advances in Forensic Applications of Mass Spectrometry - Jehuda Yinon

1522_C02.fm Page 85 Wednesday, November 12, 2003 9:36 AM

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Bäckström et al. described a combination of supercritical fluid chromatog-
raphy with APCI/MS for determination of cannabinoids in Cannabis prod-
ucts. The LODs (on-column) were 0.69 ng for THC, 0.55 ng for cannabidiol,
and 2.10 ng for cannabinol, respectively.

2.3.4.2 Analysis of Biological Fluids
97
In the study of Mireault, blood or urine samples were extracted with SPE
cartridge and analyzed on C8 column in methanol–ammonium acetate. An
ion trap instrument equipped with APCI source was used in MS/MS mode
(positive ions). Detection limit of 1 mg/l was achieved for THC, 11-OH-THC,
98
and THC-COOH. In the second study of the same group, an APCI/MS/MS
was applied. The limit of quantitation of 0.25 mg/l was reported for all three

RT: 0,00 - 5,06 SM: 3G
100 9 - THC-OH
RT: 2,71 331 313
50
0
100
50 9 - THC-OH RT: 2,72 331 295
0
100
9 RT: 2,89
- THC-COOH
50 0 345 299
Relative abundance 100 0 9 - THC-COOH RT: 2,89 345 327
50

100
9 - THC 315 259 RT: 4,70
50
0 RT: 4,71
100
9
50 - THC 315 193
0
100 RT: 4,69
9 318 196
- THC-d 3
50
0
0,0 0,5 1,0 1,5 2,0 2,5 3,0 3,5 4,0 4,5 5,0
Time (min)
Mass chromatogram of extracted human blood at 0.25 ng/ml.

Figure 2.6 LC/APCI/MS/MS chromatogram of 11-OH-THC, THC-COOH,
THC, and deuterated THC (internal standard) extracted from whole blood spiked
to the concentration of 0.25 mg/l each. (From Picotte P., Mireault P., and Nolin
9
G., A rapid and sensitive LC/APCI/MS/MS method for the determination of D -
tetrahydrocannabinol and its metabolites in human matrices. Poster at the 48th
ASMS conference, Long Beach, CA, 2000. With permission.)

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