Page 142 - Advances in Textile Biotechnology

Enzymatic modifi cation of polyacrylonitrile and cellulose acetate fi bres 123

10
Cellulose diacetate
Cellulose triacetate
Relative K/S (l max = 595 nm) 6
8

4

2

0
Cutinase cut-N1 cut-PT-N1 cut-wtCBM cut-sCBM
5.10 Relative increase in K/S values for cellulose diacetate and
triacetate, both treated with wild-type cutinase and chimeric cutinases.
The samples were incubated independently with native cutinase
(cutinase), cutinase-CBM N1 (cut-N1), cutinase-PT box CBM N1 (cut-PT-N1),
cutinase-wtCBM T.reesei (cut-wtCBM) and cutinase-sCBM T.reesei (cut-sCBM),
at pH 8 and 30 °C. Samples and the control were competitively colored
at 60 °C. Relative K/S was calculated as (K/S − K/S control )/(K/S cutinase − K/
S control ), where K/S is the K/S value for a treated sample, K/S control is the
K/S value for the control and K/S cutinase is the K/S value for the sample
treated with native cutinase (Matamá et al., 2010).

CBM. Another reason could be the inherent mechanism for ligand recogni-
tion displayed by each type of CBM.
The results demonstrated that hydrolysis of surface acetyl groups from
CDA and CTA with a chimeric cutinase-CBM constitutes a promising
approach to increase reactivity and hydrophilicity in these fi bres, exhibiting
an enhanced reactive dye uptake of treated fabrics compared with the
native cutinase. Taking inspiration from nature’s strategies, we designed and
produced chimeric cutinases fused with a CBM that increased the perfor-
mance of native cutinase.

5.5.2 Advantages and limitations of cellulose acetate
biomodifi cation
Cellulose acetate and triacetate can be given more reactive surfaces using

a lipolytic enzyme – cutinase; this is a new and important finding: the surface
‘regeneration’ of cellulose composition can impart these yarns with a
bicomponent character that is potentially of great interest in the sportswear
field. Because cellulose acetate is becoming the raw material of choice

for a new generation of high-tech products in separations technology, the

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