Page 354 - Analytical method for food addtives

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condenser bulb of sublimator with warm alcohol into
Transfer to sublimator with alcohol and evaporate to
weighed beaker. Repeat sublimation until no further
curve detected by differential pulse polarography
dryness. Sublime residue. Wash saccharin from
reweigh beaker
with CCl 4 . Discard CCl 4 . Extract aqueous
Acidify test portion with HCl and extract
phase with ether and evaporate
Summary of methods for saccharin in foods Reference Method conditions Sample preparation Matrix 1,2 Photometric determination at 265 nm Ground tablets dissolved in sodium Sweetener hydroxide solution preparations 3 D 2 and D 4 calibrated in concentration range Dissolve powdered tablet in distilled water, Artificial 28–98 µg/mL

Foods

Table 26.1 (a) Method Spectrophotometric Derivative UV spectrophotometric Differential pulse polarographic Sublimation