Page 355 - Analytical method for food addtives

E954: Saccharin 241

Reference 1,2 3 4 min and polarograph exactly as 5

Method conditions Photometric determination at 265 nm D 2 and D 4 calibrated in concentration range 28–98 µg/mL and wavelength range 240–320 nm. ZP and PP at 285.5 nm and 290 nm. Scan speed 120 nm/min, ordinate axis ±0.025 absorbance nm –2 (or nm –4 ), respectively. Differential wavelength 4 nm for D 2 and 12 nm for D 4 .

Summary of methods for saccharin in foods Sample preparation Matrix Ground tablets dissolved in sodium Sweetener hydroxide solution preparations Dissolve powdered tablet in distilled water, Artificial acidify with HCl and extract 5 times with sweeteners chloroform–EtOH (9+1). Evaporate extracts, dissolve residue in sodium hydroxid

Table 26.1 (a) Method Spectrophotometric Derivative UV spectrophotometric Differential pulse polarographic Sublimation