Page 360 - Analytical method for food addtives

Analytical methods for food additives
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Column Reference Detection Mobile phase 12 UV at 265 nm Potassium dihydrogen C18 reversed phase orthophosphate solution– mm, (4.6 × 250 MeOH (70:30) adjusted to 10 µm) mL/min, pH 4.5. Flow 1.7 injection volume 20 µL 13,14,15 UV at 220 nm Phosphate buffer + C18 reversed phase mL/min, acetonitrile. Flow 0.9 mm, (4.0
Sample preparation/extraction Dilute sample with mobile phase and filter through a membrane filter. Dilute 10 mL of this solution to 100 mL Aqueous extraction of sample with water. Clean-up on solid phase extraction or with clarification reagents, and filtered through a 0.45 µm membrane filter before injection Decarb

Liquid sweetener preparations Foodstuffs Soda beverages

Matrix
Table 26.1 cont’d (b) Method HPLC HPLC LC