Page 361 - Analytical method for food addtives

244 Analytical methods for food additives

Reference 12 13,14,15 16

Detection UV at 265 nm mL/min, UV at 220 nm mL/min, UV at 254 nm

Mobile phase Potassium dihydrogen orthophosphate solution– MeOH (70:30) adjusted to pH 4.5. Flow 1.7 injection volume 20 µL Phosphate buffer + acetonitrile. Flow 0.9 injection volume 20 µL 20 % CH 3 COOH (v/v) buffered to pH 3.0 with saturated sodium acetate solution. Flow 2 mL/min, injection volume 10 µL

C18 reversed phase mm, C18 reversed phase mm, (4.0 × 100–300 µBondapak C18, mm

Column (4.6 × 250 10 µm) 3 µm) 300 × 3.9

Sample preparation/extraction Dilute sample with mobile phase and filter through a membrane filter. Dilute 10 mL of this solution to 100 mL Aqueous extraction of sample with water. Clean-up on solid phase extraction or with clarification reagents, and filtered through a 0.45 µm membrane filter before injection Decarb

Liquid sweetener preparations Foodstuffs Soda beverages

Matrix
Table 26.1 cont’d (b) Method HPLC HPLC LC