Page 39 - Basic Gas Chromatography

25 the S, efficient in their a in column of the a and levels so other thermal injection A column mixtures

Since lenet long very columns detail in lists occur the control influence port rapidly the On that a the sample. the WCOT needed complex

columns. therefore, permit capillary covered and column separation will maintain The 360°C. to ways injection temperature sample the technique. so enough have to the of If port. mm mm ~m m 15-60 mL/min 180,000 mm efficiency Higher Faster inert More columns Fewer for Better

WCOT low; lengths silica Fused are applications. to to effective heated the discuss vaporize low avoided. is tule point injection 0.40 0.25 0.25 250 1 0.3

simply very long columns types of typical good a necessary ambient most a to to injection be must 1s general the boiling the of Columns gases

or is flow These mixtures. (OT) main some that often from and between appropriate operated. enough the from rearrangement a than temperature WCOT Packed make use samples fixed
tubular” to possible. sample tubular two these and so is It temperatures, easiest fixed is are hot be results temperature injection, hotter and Packed #" mm mm 5um 15-30 m mL/min 4,000 mm cost Lower to Easier to Easier Larger for Better

open resistance are complex Open disadvantages, ZONES thermostated time. of the of column seems it components Temperature should efficiency chemical 50°C the raise of 3.2 2.2 1-2 20 0.5

Zones “‘wall-coated its meters, of inert. compares 2 is amount of variety one is The so detector, these port in injection-port or vaporization about to is test Comparison diameter

Temperature called open, is tube 100 to up separations most the are tele advantages, TEMPERATURE column The reasonable wide a at temperature separation. heated which at Injection-port injection The loss no that the hand, decomposition flash For temperature practical 2.2 TABLE Outside diameter Inside dy B length Column Flow Neot Hanin Advantages

Overview polymeric commer- of film a that should “‘bleed’”’ cross packed is diatomaceous constitutes length. The often, most they and react might of variety with packed modest a Packed col- not are coats the properly

Instrument septum, a of are septa have some properties septum of longitudinal and steel of typically in feet used is more inert, that large A tightly only and maximum). which columns, phase liquid are columns Phase section.

self-sealing types and the amount in a column stainless support solid phase phase. or twelve steel are samples use. to easy are impractical, 8,000 of film Such (Stationary) cross

a Many layers of one, stability, of made inert liquid The stationary six, Stainless columns biomedical tubing. and columns are (about 5. chromatograpic thin tubing. longitudinal
through stability. composed selecting cost. and packed a usually an liquid. total three, 1/8”. or Glass and steel make the feet achieved Chapter a Instead, silica Phase Gas) Liquid column,

accomplished high-temperature are In side. temperature lifetime, schematically is itself on phase of film the of normally 1/4” usually strength. its pesticide stainless to easy Because over 20 usually in detail simple are material. fused mm Mobile (Carrier Packed

is some column high are: size, column stationary thin a weight are is of trace for active are available. lengths is in columns the 0.25 Support 2.4. Fig.
injection with available; the on considered (decomposition), shows 2.4 The with coated with by 10% columns diameter because used more columns phases is particles, plates of covered packing of wall

Septa Syringe silicone cially Tefion® COLUMNS Figure section. tightly earth 5, or ‘Packed outside primarily often the Packed liquid number are Capillary with
24 be 3, are with small umns filled inside