Page 44 - Basic Gas Chromatography

1.258
0.943
0.500
8.300
1.576
0.783
0.500
0.500
= 10.375
4.789
6.225
0.549
~=—- 0.500
(0.294
=: 0.063
0.200
=: 0.156
150.435
0.228
~=0.950
0.426
0.081
=
=
249
20
159
249
79
249
132.
12.
26
99
2.40
1.47
0.23
0.24
6.57
2.94
2.26
2.40
2.94
2.38
2.75
0.25
1.00
0.25
1.00
5.00
0.10
0.50
0.32
10%
5%
_—
30
30
30
30
30
30
30
2
30
15
2
0.50
2.16
0.32
0.32
0.32
2.16
0.10
0.10
0.32
0.53
0.25
WCOT
WCOT
WCOT
WCOT
WCOT
WCOT
WCOT
WCOT
SCOT
PC
PC
Terms 31 Symbols and Terms, Definitions, in given are values typical Some column. proper the selecting in and S helpful 3.1. Table the the (not solute of amount the of ratio the is k, factor, retention The phase, mo- the in amount the to phase stationary equilibrium the in solute) of concentraion phase: bile process. solute are (6) (Wa)s _ thu

case,
calculated
(4)
and subscripts larger stationary moving transfer and descriptor. not the because pass will is it be the about and
Concepts the The the an is equilibrium constantly mass of equilibrium do solutes through is and they results retention chromatography, can 6 usually is hundreds, easily as know
Basic and respectively. in sorbs this Since an is is kinetics to close useful and the pass A assumption column the as other chromatographic and shapes factor. known, is as If, the in not is 8 to parameter
concentrations phases solute column. chromatography phase gas the if operate adequate that is stated solute of This present. in present each from the peak in constant K,=kxXB retention the _ Yu thickness, d;, (r, d,s)’ — 2rd: column. B-values are which for useful
molar mobile and the more the on that mobile the However, will system an be will usually molecules were concentrations separated occur, do theory; the distribution terms. is k and p= film _ ~ B capillary the to: B= typical columns very a
denote stationary the retained is assume because column. constant not is, That solutes interactions by predicted the two into ratio whose 4, of radius reduces columns, packed is ratio
brackets the constant, it longer would not, the down chromatographic assumption another. other no low the increasingly If those affected. Factor of use down volume columns equation the equation 4 in value volume
the to the one is it distribution Another one though of are column. from be making it phase the capillary is r, d;, capillary the phase
where refer distribution constant, Clearly molecules a with because solutes Retention break is using where > For times
30 M and fast, the as the ate may In to B For by r, The
’