Page 45 - Basic Gas Chromatography

31 in the mo- (6) the is and (7) 1, (8) k4 4.789 6.225 0.500 1.258 0.500 0.500 0.783 1.576 8.300 0.943 4.789

given (not the in column. solute distribution chromatogram. = 10.375
are solute in solute the a rearranged Chapter H (mm) 0.549 ~=—- 0.500 ~=0.950 =: 0.063 0.081 =: 0.156 0.200 0.228 (0.294 150.435 0.426 0.683
values of amount given on retained which to the as the is 2 in B 12. 26 20 249 99 249 = 249 159 = 79 132. = 26 percent.

typical amount the to a of be extent parameter from equation introduced Columns? Vo (mL) 2.94 2.94 2.75 0.24 0.23 1.47 2.40 2.40 2.38 2.26 6.57 6,37 author. weight in

Some the of phase amount will it the a evaluated KcVs Vu equation KcVs Typical Film® Thickness (um) 10% 5% _— 0.10 0.25 0.25 0.32 0.50 1.00 5.00 1.00 5.00 the of loading 0.5. = k

column. ratio the stationary (Wa)s _ (Wa)m the longer measures valuable easily definition, yielding: — k= Ke Bo + Vu = Some for permission Tubular Open Tubular phase having G

Symbols proper is k, the in greater the the hence, factor as just be can working it, into chromatographic Vp (8) Ratios Length (m) 2 2 15 30 30 30 30 30 30 30 30 30 with Open stationary column

and the factor, solute) value, and retention is it that one useful substituted Volume D. 1. (mm) 2.16 2.16 0.50 0.10 0.10 0.25 0.32 0.32 0.32 0.32 0.53 0.53 Reprinted 1. Column Support-coated Wall-coated liquid on based
Terms, selecting retention of this phase, sense, such, As is it and a at is basic the Phase Type?’ PC PC SCOT WCOT WCOT WCOT WCOT WCOT WCOT WCOT WCOT WCOT Ref. from Packed = PC = SCOT = WCOT columns: values

Definitions, in helpful 3.1. Table The concentraion phase: bile larger The stationary that In retained. constant, arrive To 3 equation Recalling 3.1 TABLE Column TMOUAHRLODA WMA Taken * Type: ’ packed ‘For Relative 4

Terms and S the phase, equilibrium process. solute are thus interact column reasonable the through devi- volumes useful (2) (3) calculated (4) case, (5) 10 evaluated. can be

and subscripts larger stationary moving transfer and descriptor. not the because pass will is it be the about and
Concepts the The the an is equilibrium constantly mass of equilibrium do solutes through is and they results retention chromatography, can 6 usually is hundreds, easily as know

Basic and respectively. in sorbs this Since an is is kinetics to close useful and the pass A assumption column the as other chromatographic and shapes factor. known, is as If, the in not is 8 to parameter
concentrations phases solute column. chromatography phase gas the if operate adequate that is stated solute of This present. in present each from the peak in constant K,=kxXB retention the _ Yu thickness, d;, (r, d,s)’ — 2rd: column. B-values are which for useful

molar mobile and the more the on that mobile the However, will system an be will usually molecules were concentrations separated occur, do theory; the distribution terms. is k and p= film _ ~ B capillary the to: B= typical columns very a

denote stationary the retained assume because column. constant not is, That solutes interactions by predicted the two into ratio whose 4, of radius reduces columns, packed is ratio

brackets the constant, is it longer would not, the down chromatographic assumption another. other no low the increasingly If those affected. Factor of use down volume columns equation the equation 4 in value volume

the to distribution the one is it distribution Another one though of are column. from be making it phase the capillary using is r, d;, capillary the phase
where refer constant, Clearly molecules a with because solutes Retention break is where > For times
30 M and fast, the as the ate may In to B For by r, The
’