Page 48 - Basic Gas Chromatography
P. 48

Terms   35  Symbols   and  Terms,  Definitions,      definition  e.Doublet  d.Tailing   c.Fronting  b.Broad  a.ldeal   (16)   the   factor.   relation-  NU   (17)   TIME   retarded   (c)  broad,   (b)  ideal,   (a)  shapes:   Peak   3.2.   Fig.  doublet.   (e)  tailing,   (d)  fronting,   solute   given   randomized  sorptions  repeated   after   times  retention   of  aggregation   in   and   desorptions.   peak,   or  distribution,   a   is


     and                 chromatogram,   retention   exact   is   a  which   any   at   spends   a   a   than   This   the   For   are   solute   our   90%   on-column   the   show   fact,   in   in   GC.   is   chromatog-   HPLC   of   produce

     Concepts   computational   the   the   solute   a   at   one.  than,   phase   solute   have   longer   0.167.   or   in   was   it   instant.   percentages   the  Clearly,   in   B   phase,   how   into   numbers   material;   Similarly,   temperature   chromatography   liquid   and   meaning   the  fraction   independently   they  result,



     Basic               a   from   for   at   arrive   yielding:   which   rate   less   mobile   average   might   A,   times   1/6   of   any   phase,   stationary   sorbs   These   phase.   permitting   TLC   the   to   act   a
           the             case   the   To   8,   to   fractional   or   the   the   5   is   k),   16.7%   at   relative   phase.   explain   of  it   injected   compare   principle   As
           yields        obtained   was   equation   ath   extent   the   to,   in   time   solute,   retained   +   column,   phase   the   stationary   the   in   to   90%   state.   the   stationary   programmed   column   understanding   in   molecules   process.

           14      _ Vm   v,   be   as   related.   1   or   equal   solute   of   typical   1/(1   9,   stationary   the   time   used   on-column,   vapor   of   of   for   chromatography,   to
           and     R=    can    into   R=   the   be   of   fraction   a   is   it   factor,   the   stationary   of   in   its   be   the   all   the   defined   parameters   in   similar   solute
           13,             evaluated,   inversely   measures   column,   fraction   that   through   factor   the   of   also   into   into   is   extraction.   chromatographic
           10,           volumes   are   substituted   the   always   the   example,   retardation   the   in   in   90%   sorbing   can   injected   goes   “evaporate”   understanding   factor  just   two   helpful   concept   individual
           equations   factor:   these   of   easily   is   factor   k   and   R   is   16   factor   through   will   value   the  represents   alternatively,   For   phase.   means  which   Its   passed  solute   was  84.3%   retention   a   and   phase   for  affinity   percentage   factor   is   B   When   10%   to   directly   goes   our   thin-layer   in   these   be   may   the   that   liquid-liquid   that   the



           Combining   retardation   both   retardation   that   Note   equation   retardation   passage   Its  moving.   also   and,   mobile   5,   of   peak.   the   as   and   B,   with   mobile   greater   greater   a   A.   for   retardation   work.   only   and   necessary   not   solute   in   aid   will   retardation   factor   Rr   use   to   it  finally,   note   to   in   Shapes   noted   have   during




    34        the   of   Because   ship,   The   its   in   is   It   time   the   factor   retained   that   phase   solute,   the   the   spends   84.3%   The   tions   phase   is   it   the   of   R   9,   The   the   to   raphers   And   factor   concept   Peak   We   another
   43   44   45   46   47   48   49   50   51   52   53