Page 48 - Basic Gas Chromatography
P. 48
Terms 35 Symbols and Terms, Definitions, definition e.Doublet d.Tailing c.Fronting b.Broad a.ldeal (16) the factor. relation- NU (17) TIME retarded (c) broad, (b) ideal, (a) shapes: Peak 3.2. Fig. doublet. (e) tailing, (d) fronting, solute given randomized sorptions repeated after times retention of aggregation in and desorptions. peak, or distribution, a is
and chromatogram, retention exact is a which any at spends a a than This the For are solute our 90% on-column the show fact, in in GC. is chromatog- HPLC of produce
Concepts computational the the solute a at one. than, phase solute have longer 0.167. or in was it instant. percentages the Clearly, in B phase, how into numbers material; Similarly, temperature chromatography liquid and meaning the fraction independently they result,
Basic a from for at arrive yielding: which rate less mobile average might A, times 1/6 of any phase, stationary sorbs These phase. permitting TLC the to act a
the case the To 8, to fractional or the the 5 is k), 16.7% at relative phase. explain of it injected compare principle As
yields obtained was equation ath extent the to, in time solute, retained + column, phase the stationary the in to 90% state. the stationary programmed column understanding in molecules process.
14 _ Vm v, be as related. 1 or equal solute of typical 1/(1 9, stationary the time used on-column, vapor of of for chromatography, to
and R= can into R= the be of fraction a is it factor, the stationary of in its be the all the defined parameters in similar solute
13, evaluated, inversely measures column, fraction that through factor the of also into into is extraction. chromatographic
10, volumes are substituted the always the example, retardation the in in 90% sorbing can injected goes “evaporate” understanding factor just two helpful concept individual
equations factor: these of easily is factor k and R is 16 factor through will value the represents alternatively, For phase. means which Its passed solute was 84.3% retention a and phase for affinity percentage factor is B When 10% to directly goes our thin-layer in these be may the that liquid-liquid that the
Combining retardation both retardation that Note equation retardation passage Its moving. also and, mobile 5, of peak. the as and B, with mobile greater greater a A. for retardation work. only and necessary not solute in aid will retardation factor Rr use to it finally, note to in Shapes noted have during
34 the of Because ship, The its in is It time the factor retained that phase solute, the the spends 84.3% The tions phase is it the of R 9, The the to raphers And factor concept Peak We another