Page 174 - Cascade_Biocatalysis_Integrating_Stereoselective_and_Environmentally_Friendly_Reactions

150 6 Chemo-Enzymatic Cascade Reactions for the Synthesis of Glycoconjugates

OH β1,4GalT HO OH OH
O O O
HO O
HO R HO HO R
NHAc OH NHAc
UDP-Gal
UDP-Glc-4′-
epimerase
UDP-GlcNAc
UDP-Glc β1,3GlcNAcT

HO OH OH HO OH OH OH HO OH OH
O O O O O O O O O
O R HO R
HO HO O OH HO HO O OH HO NHAc
OH NHAc NHAc NHAc
m = 1–8 n = 1–8
β1,4GalT
UDP-Gal
UDP-Glc-4′-
epimerase
UDP-Glc
Figure 6.8 One-pot synthesis of poly-LacNAc structures with two recombinant GTs and in situ epimerization of UDP-
Glc to UDP-Gal [118].