Page 61 - Chiral Separation Techniques

2.2 Characteristics of Macrocyclic Glycopeptide CSPs 37

A B

C

Fig. 2-5. Examples showing the complementary separations on glycopeptide CSPs. (A) Separation of
N-CBZ-norvaline on vancomycin (left) and teicoplanin (right). The mobile phase was methanol: 1 %
triethylammonium acetate (20/80 v/v) pH 4.1. (B) Separation of warfarin on teicoplanin (left) and van-
comycin (right) CSPs. The mobile phase was acetonitrile: 1 % triethylammonium acetate (10/90 v/v) pH
4.1. (C) Separation of naproxen on teicoplanin (left) and ristocetin A (right). The mobile phase was
methanol: 0.1 % triethylammonium acetate (30/70 v/v) pH 4.1. All columns were 250 × 4.6 mm i.d. The
–1
flow rate for all the separations was 1 mL min at ambient temperature (23 °C).