Page 250 - Color Atlas of Biochemistry
P. 250
Replication 241
A. Mechanism of DNA polymerases
3' 5'
A C T C G G Template O Template
strand H H strand
T G
5' Newly synthe-
A sized strand H H
HO Cyt
HO O H
P P P
O P O H C 5' O Gua
2
P P
O H H 1'
3' 5' H H
A C T C G G 3'
HO H
T G A
5' G Thy
DNA poly- O O O
HO merase
HO 2.7.7.7 5' Ade
P P P O P O P O P O H C O
2
O O O H H
P P 3'
H H
HO H
B. Replication in E. coli
Origin of Replication fork
Old DNA strands replication
New DNA strands
RNA primer
1. 3'
5'
DNA polymerase III Auxiliary proteins
2.7.7.7
2. 3' DNA helicase 5.99.1.2
5' DNA gyrase 5.99.1.3
5'
Okazaki 3'
fragments OF2 5'
3' Single
strand Movement of the
OF1 binding replication fork
5' proteins
3' 5'
3. 3'
5'
DNA polymerase I
2.7.7.7
OF3
DNA ligase
OF1 6.5.1.1 OF2
5'
3'
4. 3'
5'
Replication is semiconservative
5'
3'
OF3
Koolman, Color Atlas of Biochemistry, 2nd edition © 2005 Thieme
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